Gel-Based and Gel-Free Quantitative Proteomics Approaches at a Glance
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چکیده
منابع مشابه
Gel-Based and Gel-Free Quantitative Proteomics Approaches at a Glance
Two-dimensional gel electrophoresis (2-DE) is widely applied and remains the method of choice in proteomics; however, pervasive 2-DE-related concerns undermine its prospects as a dominant separation technique in proteome research. Consequently, the state-of-the-art shotgun techniques are slowly taking over and utilising the rapid expansion and advancement of mass spectrometry (MS) to provide a ...
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gel-based proteomics
proteomics has emerged since 1995 for studying the total proteome of cells, tissues, or organisms at any condition. the study of proteins has a fundamental importance because they are the functional and final molecules of the gene expression process and proteomics could be a valuable technique for achieving this purpose. mass spectrometry is a complementary technique for proteomics that allows ...
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The comprehensive analysis of gene expression in complex biological systems has demanded the development of new technologies to study the cell transcriptome and the cell proteome. Each approach has advantages and disadvantages from both the conceptual and the methodological viewpoints. Differential proteomics, the comparison of distinct proteomes (eg normal versus diseased cells, diseased versu...
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ژورنال
عنوان ژورنال: International Journal of Plant Genomics
سال: 2012
ISSN: 1687-5370,1687-5389
DOI: 10.1155/2012/494572